Modelling the effect of Apoptosis on Epithelium FLUidity

ANR-22-CE45-0028 - March 2023 / February 2027

Epithelia have a viscoelastic behaviour: they respond as solids over short times and as fluids over large times. This fluidity plays an essential role in morphogenesis and tissue deformation. At the cellular scale, fluidity is achieved by the remodelling of junctions between cells due to their interactions but also by cell division and death. However, the contribution of apoptosis to fluidity has been little studied and remains unclear since cell death is also associated with local elastic constraints. Our project first aims at developing a novel particle model, describing cell cycles and the polarities interactions (Vicsek-like model), to assess the impact of cell death rate on tissue fluidity. The construction of this model will be strongly guided by comparisons with in vitro (MDCK cells) and in vivo (Drosophila pupa) experiments. From this particle model, a hydrodynamic model will be rigorously derived and simulations based on this new macroscopic description will be utilized to improve the understanding of tissue dynamics. The present study will thus provide a generic model, consistent with the experimental data and allowing one of the first systematic assessments of the role of apoptosis on tissues.

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